【2月3日报告】Biomolecular scaffolds for enhanced biomass processing and cancer marker detection----中国科学院天津工业生物技术研究所.doc

Qing Sun Address: 150 Academy Street, University of Delaware, Newark, DE 19716 Email: sunqing@udel.edu Phone: 951-333-7856 Education University of Delaware Ph. D. candidate of Chemical and Biomolecular Engineering 2015.05(expec ted) GPA: Tsinghua University 3.94/4.0 Master of Environmental Engineering GPA: 2009.06 87.5/100 Wuhan University Bachelor of Environmental Engineering 2007.07 GPA: 89.7/100 Rank: 1/50 Bachelor of Biological Science GPA: 82.3/100 Experiences Graduate 08/2013 - present Research Assistant University of Delaware Post-translational modifications of protein-nanocages as a universal biosensor platform Advisor: Dr. Wilfred Chen Successfully assembled protein nanocage based biosensors for thrombin and cancer marker detection. Overcame limitations of conventional bio-nanoparticle surface decoration strategies by using bio-friendly post-translational modification techniques. Achieved signal amplification by immobilizing multiple copies of sensor components on the same protein nanocage. Demonstrated the modularity of input and output domains to increase the universality of the platform for multiple diagnostic and therapeutic applications.  Immobilized elastin-like polypeptide (ELP) on protein nanocages as purification tags to enable chromatography-free purification of assembled nanocages. Graduate 12/2009 – 09/2013 Research Assistant University of Delaware Artificial cellulosomes construction on DNA template for efficient cellulose hydrolysis Successfully utilized ease to synthesize DNA scaffolds as University of California, templates for artificial cellulosomes assembly through Zinc Riverside Finger Protein (ZFP) guided immobilization. Advisor: Dr. Wilfred Chen Elastin-like polypeptide was used as purification tag as well as a linker for ZFP and cellulases. Observed enhancements in cellulose hydrolysis comparable with already existing protein scaffolds based artificial cellulosomes. Targeting higher order-structure cellulosomes with DNA nanotechnology in anticipation of higher cellulose hydrolysis efficiency. Graduate 11/2009 -12/2010 Research Assistant Low-cost defluoridation adsorbents development for drinking water safety Tsinghua University Professor Zhenyu Li Focused on developing Magnesium Oxide as defluoridation adsorbents using microwave processing followed by characterization. Lab manager University of Delaware 09/2012 -10/2014 Wilfred Chen lab Supervising Chen lab with up to 10 graduate students and 2 postdoctoral researchers. Ensuring resource planning, equipment functions, purchasing and maintenances of the whole lab to provide suitable working environment. Coordinated with the UDel Environmental & Health Safety Department to identify and address potential safety issues. Skills  Technical Skills Molecular biology: Microbial protein expression and optimization; Protein purification (chromatographic and non-chromatographic); PCR; Plasmid extraction and transformation; DNA recombination; SDS-PAGE; Native PAGE gel; Western Blot; Immunofluorescence; ELISA; Protein-protein/molecular interactions; UV-VIS spectroscopy; Enzyme engineering Cell culture: Mammalian cell culture; Cell passaging; Cell fixation; Targeted drug delivery; Cell imaging  Language Skills Native in Chinese, Full professional proficiency in English Publications and Presentations  Qing Sun and Wilfred Chen. Targeted drug delivery by tetrafunctional protein nanocage assembled through post-translational modification. In preparation.  Qing Sun, Qi Chen, Daniel Blackstock and Wilfred Chen. Protein nanocage serving as a universal platform for cancer detection. In preparation.  Qing Sun, Bhawna Madan, Shen-Long Tsai and Wilfred Chen. Creation of artificial cellulosome on DNA scaffolds by zinc finger protein-guided assembly for efficient cellulose hydrolysis. Chemical Communications, 2014, 50: 1423-1425.  Qi Chen, Qing Sun, Szu-Wen Wang, Eric T. Boder and Wilfred Chen. Thermally-responsive and biocatalytic protein nanocages by sortase-mediated stepwise modular display. In preparation.  Daniel Blackstock, Qing Sun, and Wilfred Chen. Fluorescent protein-based molecular beacons by zinc finger protein-guided assembly. Biotechnology and Bioengineering. Accepted for publication.  Ka-Hei Siu*, Rebecca Chen*, Qing Sun*, Long Chen*, Shen-Long Tsai* and Wilfred Chen. Molecular scaffolds for pathway enhancement. Current Opinion in Biotechnology. In preparation. (*equal contribution)  Miso Park, Qing Sun and Wilfred Chen. Positional assembly of enzymes on bacterial outer membrane vesicles for cascade reactions. PLoS ONE 9(5): e97103. doi:10.1371/journal.pone.0097103.  Rebecca Chen*, Qi Chen*, Heejae Kim*, Ka-Hei Siu*, Qing Sun*, Shen-Long Tsai* and Wilfred Chen. Biomolecular Scaffolds for Enhanced Signaling and Catalytic Efficiency. Current Opinion in Biotechnology. 2014, 28:59–68. (*equal contribution) Qing Sun sunqing@udel.edu 951-333-7856  Daniel Blackstock*, Miso Park*, Qing Sun*, Shen-Long Tsai* and Wilfred Chen. Engineering protein modules for diagnostic applications. Current Opinion in Chemical Engineering, 2013, 2: 416-424. (*equal contribution)  Heejae Kim*, Qing Sun*, Fang Liu*, Shen-Long Tsai* and Wilfred Chen. Biologically assembled nanobiocatalysts. Topics in Catalysis, 2012, 55: 1138-1145. (*equal contribution)  Qing Sun, Shen-Long Tsai, Bhawna Madan and Wilfred Chen. Functional self-assembly of artificial cellulosomes for efficient cellulose hydrolysis. AIChE, October 2012, Pittsburgh, Pennsylvania.  Qing Sun, Qi Chen, Eric T. Boder, Szu-Wen Wang and Wilfred Chen. Post-translational modification of artificial cellulosomes for efficient cellulose hydrolysis. AIChE, November 2013, San Francisco, California.  Qing Sun, Qi Chen, Szu-Wen Wang and Wilfred Chen. Post-translational modification of protein nanocages as a universal biosensor platform. ACS, March 2014, Dallas, Texas. (Poster) Teaching Experiences  Teaching Assistant. University of California, Riverside. CHE 160C Chemical Engineering Lab 03/2010-06/20 12  Guide for the Sino-Italian Tsinghua Environmental and Energy Efficient Building, China. 07/2008~09/20 08 Selected Honors and Awards  Graduate fellowship, University of California, Riverside USA 09/2009  Third Prize of Summer Employment Practice, Tsinghua University China 08/2008  Second Prize at the 6th Challenge Cup, National and Hubei Provincial Student Extracurricular Academic Science and Technology Work Competition China  06/2007 4 years Outstanding students, Outstanding Undergraduate Thesis and Graduate, Wuhan Name: University Email: Phone: Qing Sun sunqing@udel.edu 951-333-7856 China 06/2007